An Improved Brome mosaic virus Silencing Vector: Greater Insert Stability and More Extensive VIGS
نویسندگان
چکیده
منابع مشابه
Longidorus breviannulatus as a Vector for Brome Mosaic Virus.
Longidorus breviannulatus N o r t o n & Hoffmann, 1975 was described from corn in Iowa. Brome mosaic virus (BMV) was initially isolated in the Great Plains area of the United States from perennial brome grass, Bromus inermis. Infrequent localized outbreaks of BMV in barley were believed to result from mechanical inoculation by farm machinery moving across infected brome grass and into barley fi...
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Background and Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense mechanism. By infecting plants with recombinant viruses containing host genes inserted in the viral genome, VIGS achieves the RNA silencing process. The purpose of this study was to investigate the possibility of tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana be...
متن کاملSugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana
Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...
متن کاملCharacterization of a Brome mosaic virus strain and its use as a vector for gene silencing in monocotyledonous hosts.
Virus-induced gene silencing (VIGS) is used to analyze gene function in dicotyledonous plants but less so in monocotyledonous plants (particularly rice and corn), partially due to the limited number of virus expression vectors available. Here, we report the cloning and modification for VIGS of a virus from Festuca arundinacea Schreb. (tall fescue) that caused systemic mosaic symptoms on barley,...
متن کاملEfficient Amplification of Brome Mosaic Virus
The genome of brome mosaic virus (BMV) is divided among messenger polarity RNA1, RNA2, and RNA3 (3.2, 2.9, and 2.1 kilobases, respectively). cis-Acting sequences required for BMV RNA amplification were investigated with RNA3. By using expressible cDNA clones, deletions were constructed throughout RNA3 and tested in barley protoplasts coinoculated with RNA1 and RNA2. In contrast to requirements ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2017
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.17.00905